Figure 6

The miR-19a-19b-20a sub-cluster modulates the global transcriptome landscape of activated fibroblasts in fibrotic lungs. (A,B) Gene-expression profile of GFP⁺ hCD271⁺ intratracheally transferred lung fibroblasts at day 3 post-transfer. Intratracheal transfer was performed on day 7 post-bleomycin administration. Heat map represents the (A) downregulated and (B) upregulated genes in the sub-cluster-fibroblasts. Each column represents an miRNA group, and each row represents an individual gene. (C) qPCR validation of Adam12, Ctgf, Itga5, Dcn, Igfbp5, Mmp3, and Spp1 expression in GFP⁺ hCD271⁺ intratracheally transferred lung fibroblasts at day 3 post-transfer. Intratracheal transfer was performed on day 7 post-bleomycin administration. (D) GO analysis of 160 differentially expressed genes using DAVID version 6.8. Significantly enriched GO terms and associated genes are shown. (C) Graphs show the mean ± SEM [n = 6 (control) and n = 5 (sub-cluster)]. *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t-test. Cohen’s effect size d in these data was >1.3. See also Supplemental Table S5.