Figure 3
From: PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells
Silencing of PIWIL2 and PIWIL4 decreases piRNA expression during ER stress. (A) 16HBE14o- cells were transfected with siRNA against PIWIL2 (siPIWIL2), PIWIL4 (siPIWIL4) or co-transfected with both (siPIWL2/4) and treated with TM (2.5 µg/ml) or ALLN (100 µM) for 6 hours, and the corresponding changes in PIWIL2 and PIWIL4 protein levels were detected with SDS-PAGE and Western Blot and normalized to the β-Actin (2 µg of total protein per lane). The experiments were repeated twice and the error bars represent standard errors. Significant changes (p < 0.05) are marked with an asterisk. The original blots and gels are provided in Supplemental Fig. 4. (B) Reduction of PIWIL2 and PIWIL4 relative expression with siRNAs (siPIWIL2/4) lowers piRNAs levels after 6 hours of ER stress. The piRNAs levels were accessed by next generation small RNA sequencing and expressed as fold change relative to the no stress control. The groups (ALLN and ALLN siPIWIL2/4; TM and TM siPWIL2/4) were compared using ANOVA on ranks. Significant changes (p < 0.05) are marked with an asterisk.
