Figure 4
From: Multigenerational impacts of bile exposure are mediated by TGR5 signaling pathways
Paternal CA exposure alters global DNA methylation levels in sperm cells. (a) Global DNA methylation levels in spermatozoa of F0CT;+/+, F0CA;+/+; F0CT;−/−; and F0CA;−/− mice. (b) Pie charts of the relative percentage of hyper- or hypo- DMRs in spermatozoa of F0CT;+/+, F0CA;+/+; F0CT;−/− and F0CA;−/− mice. (c) Volcano plots of comparisons of all DMR assessed in RRBS analysis of spermatozoa from F0CT;+/+, F0CA;+/+; F0CT−/−; and F0CA;−/− mice. (d) Representation of specific and common differentially methylated sequences in F0CT;+/+, F0CA;+/+; F0CT;−/−; and F0CA;−/− mice. RRBS was performed on 1 experiment on 3 samples per group. Each sample is a pool of 3 individual mice. For a data are expressed as means +/− SEM. n = 8–12 per group from 3 independent experiments. *p < 0.05 vs. control diet group.
