Figure 5
From: Multigenerational impacts of bile exposure are mediated by TGR5 signaling pathways
F1CA;+/+ males have altered BA metabolism. (a) Liver mRNA accumulation of Cyp46a1 and Cyp7a1 normalized to β-actin mRNA levels in 15-days old F1CT;+/+, F1CT;−/−, F1CA;+/+ and F1CA;−/− mice. (b) Liver mRNA accumulation of Cyp46a1 and Cyp7a1 normalized to β-actin mRNA levels in 15-days old F2CT;+/+ and F2CA;+/+ mice. (c) Liver mRNA accumulation of Kcnq1 normalized to β-actin mRNA levels in 15-days old F1CT;+/+, F1CT;−/−, F1CA;+/+ and F1CA;−/− mice. (d) Liver mRNA accumulation of Kcnq1 normalized to β-actin mRNA levels in 15-days old F2CT;+/+ and F2CA;+/+ mice. (e) Liver mRNA accumulation of G6Pase and Pepck normalized to β-actin mRNA levels in adult F1CT;+/+, F1CT;−/−, F1CA;+/+ and F1CA;−/−mice. (f) Liver mRNA accumulation of G6Pase and Pepck normalized to β-actin mRNA levels in 15-days old F2CT;+/+ and F2CA;+/+ mice. Data are expressed as means +/− SEM. n = 8–12 per group from 3 independent experiments. *p < 0.05 vs. control diet group.
