Table 1 Primers used in the study.
From: CRISPR/Cas9-mediated mutagenesis of homologous genes in Chinese kale
Primer names | Sequence of primers (5′-3′) | Aims |
|---|---|---|
sgRNA-BaPDSs-F | GATGGAGATTGGTATGAAAC | Synthesis of the target site |
sgRNA-BaPDSs-R | GTTTCATACCAATCTCCATC | |
BaPDS1-CRISPR test-F | CCTGCAAAGCCTTTAAAAGTTGTCATT | Detection of the mutation in transgenic plants |
BaPDS1-CRISPR test-R | CCAAGTTCTCCAAATAAGTTCTGCACG | |
BaPDS2-CRISPR test-F | CCTGCAAAGCCTTTAAAAGTTGTGATC | |
BaPDS2-CRISPR test-R | GCTATAGAAGATAAGAGCCGAGCCT | |
Hyg-F | CGATTGCGTCGCATCGACC | Detection of the hygromycin resistance gene |
Hyg-R | TTCTACAACCGGTCGCGGAG | |
Off-target1-F | ATTCCTTGGAATTAGCTCTTCACTTGAC | Off-target analysis |
Off-target1-R | GATGGGGACTCGAATCTTATCTGCC | |
Off-target2-F | CAGGAATGCATGGGAAAGCATGAATG | |
Off-target2-R | ATGATGCAACCGGGTAGTTTAATCG | |
Off-target3-F | CATCATGAGTGGGGACAAGTTGTG | |
Off-target3-R | TCAGCGTCCATGAGAGGTAAAGGG |
