Table 3 Potential off-target analysis.
From: CRISPR/Cas9-mediated mutagenesis of homologous genes in Chinese kale
Target | Sequence | Mismatches | Efficiency |
|---|---|---|---|
Target site | GATGGAGATTGGTATGAAACCGG | ||
Off-target site1 | TATAGTGATTAGTATGAAACGGG | 4 | 0.0% |
Off-target site2 | AAAGGAGATTGCAATGAAACTGG | 4 | 0.0% |
Off-target site3 | GGTGGAGCTTGTTATGAAACTGG | 3 | 0.0% |
