Figure 6
Sequence analysis of candidate targets identified from SWATH proteomics. A partial list of candidate targets of miR-26a identified by proteomics (a) and verification of miR-26a targets by luciferase assays (b). The transcripts of all genes identified by proteomics were searched against nucleotide sequence databases to obtain their 3ʹ-UTR sequences. Several computational programs were employed to predict potential targets of miR-26a, which were then compared with our proteomic data. Luciferase assays were performed to verify miR-26a candidate targets identified by our proteomics. Data are relative luciferase activities normalized to the corresponding transfections with control oligo and shown as the mean ± SD of five replicates. Asterisks indicate p < 0.05 using the two-tailed t-test (miR-26a mimic vs. control oligo).
