Figure 2
(A) Phylogenetic relationship and exon-intron arrangement of VaWRKY genes (including isoforms; the digit after the decimal in Gene ID indicates the isoforms). Multiple alignment of VaWRKY gene sequences was executed by Clustal W and the phylogenetic tree was created using MEGA 6.0 by the Neighbor-Joining (NJ) method with 1,000 bootstrap replicates. The exon-intron arrangement was executed using Gene Structure Display Server 2.0. The exons and introns were represented by yellow boxes and black lines. The size of introns can be estimated using the scale given at the bottom. The V-type introns in Group IIa and Group IIb genes and R-type introns present in the remaining genes were indicated as green and red closed circles below the introns, respectively. (B) Phylogenetic relationship and exon-intron arrangement of VrWRKY genes. Multiple alignment of VrWRKY gene sequences was executed by Clustal W and the phylogenetic tree was created using MEGA 6.0 by the Neighbor-Joining (NJ) method with 1,000 bootstrap replicates. The exon-intron arrangement was executed using Gene Structure Display Server 2.0. The exons and introns were represented by yellow boxes and black lines. The size of introns can be estimated using the scale given at the bottom. The V-type introns in Group IIa and Group IIb genes and R-type introns present in the remaining genes were indicated as green and red closed circles below the introns, respectively.
