Figure 1 | Scientific Reports

Figure 1

From: Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa

Figure 1

Domain organization, construct design and purification of GspL. (a) Schematic representation of GspL (blue) and its positioning in the bacterial inner-membrane (IM). FLD: ferredoxin-like domain, MPD: membrane proximal domain, CD: cytoplasmic domain. Numbering corresponds to the domain boarders, which can be traced to the sequence in panel b. Except for the cytoplasmic part, GspL shares the domain organisation with GspM (grey), its well established interaction partner. (b) Protein sequence of the construct used in the study. GspL sequence is in blue, whereas purification tag sequence is in black. Serendipitously, in the course of crystallization experiment, GspLperi was proteolytically cleaved in the middle of MPD (indicated with scissors), which lead to crystallization of the shorter construct, herein termed GspLfld to distinguish it from the initially purified recombinant GspLperi (c) SEC profile of GspLperi accompanied by SDS-PAGE analysis of the indicated peak fractions. EEP: early elution peak, LEP: late elution peak.

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