Figure 2 | Scientific Reports

Figure 2

From: Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa

Figure 2

GspLperi adopts dimeric and tetrameric forms as revealed by SEC-MALLS (a) SEC-MALLS analysis of late elution peak of GspLperi shows a dimeric form (dashed line), and the oligomeric state does not change upon concentration (solid line). (b) GspLperi from early elution peak diversifies into two populations, of tetramers and dimers, respectively (dashed line). Concentration of the EEP GspLperi does not enrich the tetrameric population (solid line). However, the procedure results in longer time separation between the purification and the light scattering experiment, which shifts the tetramer/dimer ratio in favor of dimer. Tetramer to dimer ratio calculated as the ratio of areas under the relevant peaks is 0.70 at lower and 0.57 at higher concentration. Molecular weight of GspLperi calculated from the sequence is 13.95 kDa.

Back to article page