Figure 5

SEC-SAXS analysis validates the existence of dimers and tetramers of GspLperi in solution. (a) The experimentally determined distance distribution functions of dimer and tetramer of GspLperi (solid lines, blue and black) in comparison to the calculated P(r) functions of dimer and monomer (dashed lines, green and red) of crystal form 1. (b) Ab initio envelopes generated for dimer (green) and tetramer (cyan) (c) The dimeric envelope manually fitted with the dimer of crystal form 1 and an ensemble of most populous models of the N-terminus, presented as chains of spheres. (d) Kratky plot of the GspLperi dimer.