Figure 4
The transcriptome profile of cell cycle arrest and re-entry. Ring-stage intraerythrocytic P. falciparum 3D7 parasites were arrested with DFMO (2 mM) for 24 h prior to sampling (A1–3, arrested, sampled at 0, 3 and 6 h after the 24 h DFMO treatment) or left untreated (C, control). Additionally, after 24 h DFMO pressure, the cell cycle arrest was reversed with 2 mM putrescine and sampled after 3 h (RE1), 6 h (RE2) or 12 h (RE3). (a) Correlation plot displays the global Pearson correlations within and between each of the treatment conditions. (b) Global Pearson correlation to the time-mapped published IDC transcriptomic profiles2 for the arrested samples (A1–3, blue lines), and re-entered samples (RE1–3, orange lines). Arrows are used to emphasise the peak areas of correlation for the arrested (A1–3) and each RE sample (RE1–3). Representative illustration of parasites shown below graph. Parasite drawings were modified from freely available images (https://smart.servier.com/) under a Creative Commons Attribution 3.0 Unported Licence.
