Figure 4
11A1 immunoreactivity in neurons. (A and B) Representative images were obtained under 63x objective lens. Cortical neurons of inferior parietal cortex, layer V, co-stained with 11A1 and MAP2 antibodies and 11A1, MAP2 and Cathepsin D antibodies. The rows of figures correspond to three age groups: early 30 s, middle 40 s, and early 60 s. (A) Left panels show the 11A1 signal (green), middle panels indicate MAP2 signal (red) and right panels show merged images. Scale bar 5 µm. (B) Left panels show the 11A1 signal, middle panels indicate Cathepsin D signal and right panels show merged images. The white contours in the merged images indicates the cell shape identified by MAP2. Intracellular 11A1 immunoreactivity (green) partially colocalized with Cathepsin D signal (Magenta). Scale bar 5 µm. (C) Verification of 11A1 antibody’s specificity against Aβ toxic conformer in neurons of the three age groups. 11A1 antibody was pre-incubated with (bottom panels) or without (top panels) its immunogen, E22P-Aβ9-35ox peptide, prior to incubation of tissue sections with the primary antibody. The pre-incubation with immunogen abrogates the 11A1 signal (green) in and around MAP2 signals (magenta). Images were obtained under 20x objective lens. Scale bar 20 µm. (D) Percentage of cortical neurons labeled with 11A1 in measured area across the age-spectrum (30–65 years). The relationship with age is not significant (p = 0.807). (E) Number of neurons in measured area across the age-spectrum (30–65 years). There is no significant change in the number of neurons with age (p = 0.6061).
