Figure 2
From: Cardol triene inhibits dengue infectivity by targeting kl loops and preventing envelope fusion
Preliminary cell-based screening. (A) DENV2 inhibition; Vero (5 × 104 cells) were treated with DENV2 (M.O.I. of 0.1) and 10 and 25 µM anacardic acid, cardanol, and cardol mixtures for 72 h and supernatants were analyzed by plaque titration. DMSO was used as a mock treatment referring to 100% infection. (B,C) Cytotoxicity of Vero cells at (B) 48 h and (C) 72 h were performed by treatment of Vero (5 × 104 cells) with 10 and 25 µM anacardic acid, cardanol, and cardol mixtures for 48 and 72 h. Cell viability was accessed using MTS reagents and DMSO-treated cells referred to 100% cell survival.
