Figure 1 | Scientific Reports

Figure 1

From: Identification of Two Mannosyltransferases Contributing to Biosynthesis of the Fungal-type Galactomannan α-Core-Mannan Structure in Aspergillus fumigatus

Figure 1

Structure and expression of A. fumigatus proteins Afu5g02740/AFUB_051270 (termed core-mannan synthase A, CmsA) and Afu5g12160/AFUB_059750 (termed CmsB). (A) Schematic representations of the CmsA and CmsB proteins. The vertical black bars indicate transmembrane (TM) domains of CmsA (7–26 aa) and CmsB (48–70 aa), the gray bars indicate catalytic domains of CmsA (27–397 aa) and CmsB (71–442 aa), and the dark gray bar indicates an unknown domain of CmsB (443–506 aa). 247EPD249 and 262EPN264 sequences indicate a DXD-like motif. (B) SDS-PAGE analysis of purified recombinant CmsA. Purified recombinant CmsA (4.4 µg) was separated by 5–20% SDS-PAGE and stained with Coomassie brilliant blue, revealing bands of approximately 42 kDa.

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