Figure 4 | Scientific Reports

Figure 4

From: Identification of Two Mannosyltransferases Contributing to Biosynthesis of the Fungal-type Galactomannan α-Core-Mannan Structure in Aspergillus fumigatus

Figure 4

Phenotypic analyses of WT (A1151) and core-mannan synthase disruptants. (A) Colony phenotypes of core-mannan synthase disruptants (∆cmsA, ∆cmsB, ∆cmsAB) and corresponding complementary strains (∆cmsA::cmsA and ∆cmsB::cmsB). Conidia were serially diluted and spotted (number of conidia, 106, 105, 104 and 103). Strains were grown on minimal medium at 37 °C for 36 h. (B) Microscopic analysis of WT (A1151), ∆cmsA, ∆cmsB, and ∆cmsAB conidiophores. Conidiophore morphology of WT (A1151) (a), ∆cmsA (b), ∆cmsB (c) and ∆cmsAB (d) was observed under 100X (left panels) or 400X (right panels) magnifications. Conidiophores were cultured on minimal medium for 3 days. Scale bars indicate 100 µm (left panels) or 50 µm (right panels). Arrows indicate aberrant conidiophores formed as abnormal swelling structure on the tip or side of vesicles.

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