Figure 6

Validation and differential expression analysis of genes involved in seed size/weight determination via RT-qPCR. (a) Expression profile of the genes at late-embryogenesis (S3) and mid-maturation (S5) stages of seed development in JGK 3 and Himchana 1 cultivars revealed from RNA-seq and RT-qPCR is shown in the left and right panels, respectively. (b) Differential expression of these genes at S3 and S5 stages of seed development in two moderately large desi-type (JG 11 and ICC 4958) and moderately small-seeded kabuli-type (ICC V2 and ICC 8261) chickpea genotypes via RT-qPCR analysis is shown. Color scales at the bottom represent row-wise z-score.