Figure 2
From: Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus
Fungi–specific up-regulation of activation markers. (A–C) Representative dot plots of flow cytometric analysis showing the frequency of CD4+CD45RA+ T cells of neonates (A), infants and children (C) or adults (B) expressing CD25 or CD69 after 3-day stimulation with monocytes matured with heat-inactivated C. albicans or A. fumigatus. Data are representative of at least 5 donors. (D) Bar graph showing CD25 expression of CD4+CD45RA+CD31+ T cells isolated form neonates, infants, children and adults in response to C. albicans PepMix (brown) as in (A–C). T cells were stimulated for 3 days and determined using flow cytometry. Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, as determined by one-way Anova with Tukey post hoc test.
