Figure 3

Fungi-induced T cell receptor V_ß family response. CD4⁺CD45RA⁺ T cells from neonates, infants, children, and adults were co-cultured with monocytes pulsed with C. albicans- or A. fumigatus-lysates and expression of different V_ß repertoire on T cells was measured by flow cytometry. (A–C) Frequency of V_ß9 (left panel), V_ß16 (middle panel) or V_ß17 (right panel) expressing unstimulated T cells (A), T cells stimulated for 3 days either with C. albicans (B) or A. fumigatus (C) are shown. Each dot represents a different donor. (D,E) Bar graph showing TCR-V_ß expression either by CD4⁺CD45RA⁺CD25⁺ T cells from neonates, infants, children, and adults or by CD4⁺CD45RO⁺CD25⁺ T cells from adults. T cells are stimulated for 3 days either with C. albicans (orange) or A. fumigatus (blue). Cumulative results are shown and each dot represents a different donor. The error bars in figures denote ± SD. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, as determined by one-way Anova with Tukey post hoc test (A–C) or Kruskal Wallis with Dunn’s post hoc test (D and E) (F) TCR-Vß repertoire usage in CD4 + CD45RA + T cells of infants and children as well as adults after co-culturing with monocytes pulsed with C. albicans- or A. fumigatus-lysates. The allocation of the groups took place if the expression of TCR-Vß family in the stimulated samples was higher than in unstimulated controls.