Figure 4

Reconstructed terpenoid backbone biosynthesis for Rafflesia cantleyi at transcript level. This pathway was reconstructed based on the detection of transcripts present. Of the (a) two mechanisms present for IPP production, Rafflesia is found to consist of only the (b) genes present in cytoplasm but not (c) genes in the plastid. Rafflesia-containing enzymes with EC numbers include: 2.3.1.9, acetyl-CoA c-acetyltransferase; 2.3.3.10, hydroxymethylglutaryl-CoA synthase; 2.7.1.36, mevalonate kinase; 2.7.4.2, phosphomevalonate kinase; 4.1.1.33, diphosphomevalonate decarboxylase; 2.5.1.1, 2.5.1.10, farnesyl diphosphate synthase; 2.5.1.92, (2z,6z)-farnesyl diphosphate synthase; whereas enzymes confined to the plastid were labeled with EC numbers: 2.2.1.7, 1-deoxy-D-xylulose-5-phosphate synthase; 1.1.1.267, 1-deoxy-D-xylulose-5-phosphate reductoisomerase; 2.7.7.60, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 2.7.1.148, 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase; 4.6.1.12, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; 1.17.7.1, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase; 1.17.7.3, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase; 1.17.7.4, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase; 5.3.3.2, isopentenyl-diphosphate Δ-isomerase; 4.2.3.27, isoprene synthase.