Figure 1
From: Sendai Virus Infection Induces Expression of Novel RNAs in Human Cells
Validation of differential gene expression induced by Sendai virus infection. Namalwa cells were infected with Sendai virus for 6 hours and total RNA was analyzed by RNA-sequencing or by RT-qPCR in independent samples. For each indicated RNA, RNA-sequencing counts are plotted on the graph on the left and RNA abundance from RT-qPCR on the right. Expression of virus-induced (a) previously-annotated genes and (b) previously-unannotated RNAs and (c) virus-suppressed genes was validated. RNA abundance data is representative of ≥2 replicate experiments and is shown normalized to GAPDH expression. Bars indicate average values of technical replicates (n = 3) with error bars representing standard deviation. Statistical analysis was done using a two-tailed Student’s t-test for RT-qPCR measurements (*p-value < 0.05, **p-value < 0.005).
