Figure 1 | Scientific Reports

Figure 1

From: Administration of hydrogen-rich water prevents vascular aging of the aorta in LDL receptor-deficient mice

Figure 1

Expression of p16INK4a/p21 was detected in CD31-positive ECs in the aortas of Ldlr−/− mice. Immunohistochemical staining of the ascending aorta in control-fed C57BL/6 and HFD-fed Ldlr−/− mice with anti-p16INK4a (a,c,e) and anti-p21 (b,d,f) antibodies. The aorta was coimmunostained with anti-CD31 (an ECs marker), MOMA-2 (a monocyte/macrophage marker), or anti-SMA (a marker for smooth muscle cells), and counterstained with Hoechst 33342 (blue). Dotted lines indicate the borders between blood vessel layers (intima and media). Scale bar: 50 μm.

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