Table 3 Metabolomics platforms. A brief overview of the platforms detailing volumes, sample preparation, and analytical instruments.

From: Persistent metabolic changes in HIV-infected patients during the first year of combination antiretroviral therapy

Targeted Metabolomics Platform

Volume serum used (μL)

Sample Prep Method

Analytical platform

Metabolite class coverage

Platform Targets (n)

Total

Quality control passed

% Missing data

Biogenic amine50

5 μL

Protein precipitation & AccQ-Tag derivatization

UPLC-TQMS

Amino acids, catecholamines & polyamines

100

62

0%

Positive lipid49

10 μL

Isopropyl alcohol extraction

UPLC-QToF

Lysophospholipids, phospholipids, cholesterol esters, di/triglycerides & sphingomyelins

250

147

0.02%

Oxylipins51

250 μL

Oasis HLB SPE extraction

HPLC-MS/MS

Hydroxylated fatty acids, prostaglandins & thromboxanes

120

68

7.7%

Oxidative stress52

150 μL

Butanol:Ethyl acetate liquid-liquid extraction

UHPLC-MS/MS

Low pH run

Isoprostanes, nitro-fatty acids, sphingosine & sphinganine

46

36

6.6%

UHPLC-MS/MS

High pH run

Sphingosine-1-phosphate & lysophosphatidic acids species.

  1. UPLC – Ultra Pressure Liquid-Chromatography; TQMS – Triple quadrupole mass spectrometer; QToF – Quad Time of Flight; HPLC - High-Pressure Liquid-Chromatography; UHPLC – Ultra High-Pressure Liquid-Chromatography; MS/MS – Triple quadrupole Mass Spectrometer.
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