Figure 1
From: Metabolomics and transcriptomics pathway approach reveals outcome-specific perturbations in COPD
Flow chart summarizing the omics methods and analysis. Peripheral blood mononuclear cells (PBMC) (n = 136 human subjects) and matched plasma (n = 131) were prepared and analyzed using functional genomics and metabolomics, respectively. Metabolomics samples were analyzed randomly in triplicate. Regression model fitting in R resulted in a list of statistically significant transcript probes and metabolites associated with each outcome. These significant transcript probes and metabolites were mapped to pathways using IMPaLA. Pathway p-values for metabolites and transcripts were combined by a meta-analysis using Fisher’s method to obtain a single p-value and FDR for each pathway. In the outcome tables, ‘# sig’ refers to the number of statistically significant transcripts probes and metabolites. BMI: body mass index, FEV1: forced expiratory volume in 1 second, FVC: forced vital capacity.
