Figure 4

Bar graphs showing concentration of triglycerides before and after high-speed centrifugation (20,000 × g for 30 minutes) in undiluted (PFP) and diluted (dPFP, diluted 1:5 in DPBS) samples (panel A, bar graphs are means with 1 standard deviation), and dot-plots showing correlations between plasma concentration of EVs and serum triglycerides (panel B) and plasma VLDL particles (panel C) under fasting conditions. Proton nuclear magnetic resonance (NMR) spectroscopy was used to determine mean particle sizes of the main lipoprotein classes, and EV concentration was measured by NTA.