Figure 4

Deformation of skull with malocclusion in adult T1KO mice. Analysis of WT and T1KO mice with or without malocclusion in P25W. Micro CT analysis (B) and quantification of CS chains (C,D). T1KO with malocclusion (T1KO (mal+)) revealed the small body size, malocclusion with angle class III and eye defect (A). Micro CT photographs demonstrated the curved frontonasal regions, dysraphism of cranial suture, bone thinning and severe periodontitis in T1KO (mal+) mice (B). The amount of CS in T1KO (mal+) was remarkably lower in all tissues than that of WT and of T1KO (mal−) mice (C). The total amount of CS chains was significantly lower in T1KO mice with severe abnormalities such as malocclusion, skeletal dysplasia and facial cleft, compared with a littermate T1KO (mal−) (D). The mRNA expression of collagen type 1 was significantly downregulated in T1KO (mal+) tail (E). n = 3, Student’s t-test.