Figure 5

Histological analysis of adult T1KO mice with severe abnormal phenotype. Histological analysis of craniofacial region of P25W mice (see also Fig. 4). HE (A–C) and immunoperoxidase staining for CS-A, Wnt3a, FGF2 and collagen type 1, counterstained with hematoxylin (B). Double immunofluorescent staining of CS-A (Texs Red) and collagen type 1 (FITC) (B). T1KO (mal+) mice showed asymmetrical aspect and the unilateral eyeball was missing (arrow; A). In the mesenchymal tissue of palatal mucosa, the immunopositivities of Wnt3a and FGF2 were diminished with CS in T1KO (mal+) mice (arrows), and the immunostaining intensity of collagen type 1 in the lamina propria were weak in the T1KO (mal+) palate by both bright and fluorescence imaging (B). The palatal process of maxilla and calvaria were significantly thinner in T1KO (mal+) mice than in WT and T1KO (mal−) mice (C,D). (Palate: n = 4, calvaria: n = 3), ANOVA adjusted by Fisher LSD. Scale bars, 1 mm (A); 250 μm (B, upper); 50 μm (B, lower); 40 μm (C).