Figure 7
EDS-like phenotype in adult T1KO mice. (A) Gross appearance of back skin, (B) Histological analysis using HE and azan staining, (E–G) Soft X-ray analysis. Some T1KO aged mice with or without malformation showed atrophic skin with focal alopecia at 1Y (A). The collagen fibers of the dermis and cornea in T1KO mice were disordered and aggregated, as stained in red by azocarmine G (arrows) (B). The thickness of dermis of the back skin was not different among WT, T1KO (−) and T1KO (+) mice (C). Two different tension tests, using whole mice and skin fragments of back skin, were performed (Supplementary Fig. S10). Skin extension was significantly higher in T1KO (−) mice (whole: 18.375 ± 0.479 mm, fragment: 16.0 ± 1.472 mm, n = 5) than that of WT mice (whole: 15.75 ± 1.090 mm, fragment: 8.667 ± 1.607 mm, n = 4). T1KO mice having malocclusion showed severe scoliosis (E,F) and joint laxity (G). Student’s t-test (C,D). Scale bars, 100 μm (B).
