Table 1 Light Scattering (LS) analysis of the MucR deletion mutants.

From: Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation

Protein

Theoretical molecular weight monomer

Experimental Molecular weight by Static LS

ExMw/ThMw

MucR

16024 Da

167000 (±1, 0%) Da

10.40

MucR33–142

12559 Da

132600 (±2, 0%)

10.50

MucR45–142

11330 Da

11510 (±0, 6%)

1.01

MucR57–142

9905 Da

10740 (±3%)

1.08

MucR33–142 mut

12334 Da

12690 (±0, 7%)

1.02

  1. The theoretical molecular weight of the monomer calculated by ProtParam tool (http://web.expasy.org/protparam/) is reported in the second column; the results by LS in the third column; the ratio between the experimental weights found and the monomer weights are reported in the fourth column.
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