Figure 3
From: IL-34 promotes foam cell formation by enhancing CD36 expression through p38 MAPK pathway
IL-34 had no significant influence on cholesterol efflux in BMDMs. (a) Non–dependent, ApoA-I-dependent or HDL-dependent cholesterol efflux was measured by incubating [3H] cholesterol-labeled macrophages with (+apoA-I, +HDL) or without (−apoA-I,−HDL) for 12 hr. Radioactivity in the medium was determined as a percentage of total radioactivity in the cells and medium. (b) Expression of ABCA1, ABCG1 and SR-B was examined by Western blot. (c) Quantitative data were presented of three independent experiments. (d) Quantitative real-time PCR was used to measure ABCA1, ABCG1 and SR-B mRNA levels. Data represent mean ± SD of n = 3 biologically independent experiments. *P < 0.05, **P < 0.01. Unprocessed original scans of blots are shown in Supplementary Fig. 3.
