Figure 4
From: Protein–Protein Affinity Determination by Quantitative FRET Quenching
Comparison of Kd values obtained from two approaches by the quenched donor emission or acceptor emission. (A) Kd values determined by either FRET quenching or emission at different concentrations of CyPet-SUMO1 were compared. (B) Kd values after global optimization of either FRET quenching or emission datasets. (C) Bootstrap analysis of both Kd values revealed no significant difference.
