Figure 1

Transcriptional activation potential of PRD-LIKE TFs on the 11 bp TAATCC-containing promoter motif. (A) Renilla normalized luminescence (RNL) for control and 4 × 11 bp firefly reporter tested with different early PRD-LIKE TFs,. “-” at the bottom “Promoter” line denotes co-transfection with plain firefly reporter (pGL4.25), while “4 × 11 bp” denotes four copies of the 11 bp core motif (containing TAATCC) inserted into the promoter of the firefly reporter (pGL4.25-4 × 11 bp). “-” on the X-axis (TF) denotes no co-transfection of pFastBac-based TF over-expression vector, for co-transfection the TF names are indicated. Asterisks indicate statistically significant (p < 0.05, t-test) differences of RNL compared to the without TF control (labelled “ref.”) in each promoter. (B) RNL fold change calculated from the ratio of “with” and “without” TFs. Fold change values were calculated from (A), but only using experiments, where pGL4.25-4 × 11 bp was co-transfected. (C) RNL fold change calculated from the ratio of “with” and “without” 4 × 11 bp core promoter. Fold change values were calculated from (A), but only using experiments, where TF over-expression vectors were co-transfected. (D) Homeodomain amino acid sequences of the PRD-LIKE TFs. The numbering on the top is relative to the N-terminus of the full-length human LEUTX, and on the bottom is relative to the start of homeodomain. Period, colon, and asterisk indicate degree of conservation; the scorings are ≤0.5, >0.5 and <1, and =1 in the Gonnet PAM 250 matrix, respectively. “#” indicates position 47 and 54 of the homeodomain.