Figure 5
Characterization of reactive astrocytes in 26.5–32-week-old Ins2AKITA and WT littermate controls. (a) Representative image of reactive astrocytes in Ins2AKITA and WT cerebral cortex, astrocytes (GFAP, green), vasculature (PECAM1, white) (n = 5, scale bar 50 μm). (b) Total cerebral cortex Gfap mRNA expression in Ins2AKITA (n = 6) and WT (n = 4) mice presented as fold change over Hprt reference gene expression. (c) Gfap mRNA expression from isolated cerebral microvascular fragments of Ins2AKITA and WT mice presented as fold change over Gapdh reference gene (n = 7). n.s. = not significant, Student’s t test.
