Figure 2
From: Crosstalk between SOX2 and cytokine signaling in endometrial carcinoma
SOX2 contributes to cell proliferation and cell cycle progression in endometrial carcinoma. (A) Immunoblotting (upper) and qPCR (lower) analysis to measure SOX2 expression in Ishikawa (Ish, left) and Ishikawa-02 (Ish-02, right) endometrial carcinoma cells. ***P < 0.001. (B) A clonogenic analysis to compare the growth of SOX2-high Ishikawa-02 (Ish-02, right) to SOX2-low Ishikawa (Ish, left) cells. Colonies were subjected to crystal violet staining (upper) and quantified by ImageJ analysis (lower). ***P < 0.001. (C) Immunoblotting (upper) and qPCR (lower) analysis to measure the effect of SOX2 knockdown in Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shSOX2. shSOX2#1 and shSOX2#2 target different regions of the SOX2 mRNA. ***P < 0.001. (D) A clonogenic analysis to compare the growth of Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shSOX2 for 14 days. Colonies were subjected to crystal violet staining (upper) and quantified by ImageJ analysis (lower). ***P < 0.001. (E) Trypan blue cell exclusion proliferation assay of Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shSOX2 for 4 days. ***P < 0.001. (F) Flow cytometry cell cycle analysis with propidium iodide staining of Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shSOX2. **P < 0.01; ***P < 0.001.
