Figure 3
From: Crosstalk between SOX2 and cytokine signaling in endometrial carcinoma
SOX2 regulates EGFR expression in a positive feedback loop. (A) qPCR analysis to measure EGFR expression in Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shSOX2. ***P < 0.001. (B) A luciferase reporter assay to measure EGFR promoter reporter activity in HEK293T cells transfected with EGFR-pGL3 plus the vector encoding SOX2 cDNA or empty control vector (Ctrl). Results were representative of three independent experiments and are expressed as the mean ± S.D. ***P < 0.001. (C) qPCR analysis to measure EGFR expression in Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shEGFR. shEGFR#1 and shEGFR#2 target different regions of the EGFR mRNA. ***P < 0.001. (D) A clonogenic analysis to compare the growth of Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shEGFRs for 14 days. Colonies were subjected to crystal violet staining (upper) and quantified by ImageJ analysis (lower). ***P < 0.001. (E) qPCR analysis to measure SOX2 expression in Ishikawa-02 cells transduced with lentiviral particles encoding scrambled control (SC) or shEGFR. ***P < 0.001. (F) qPCR analysis to measure SOX2 expression in Ishikawa-02 cells treated with or without EGF (10 ng/ml) for 24 hr. ***P < 0.001.
