Figure 4
From: Crosstalk between SOX2 and cytokine signaling in endometrial carcinoma
Effect of TGF-β stimulation on SOX2 expression in endometrial carcinoma cells. (A) A clonogenic analysis to compare the growth of Ishikawa (Ish) and Ishikawa-02 (Ish-02) treated with or without TGF-β (1 ng/ml) for 14 days. Colonies were subjected to crystal violet staining (left) and quantified by ImageJ analysis (right). ***P < 0.001. (B) Immunoblotting analysis to access phosphorylated-SMAD3 (p-SMAD3) level in Ishikawa (Ish) and Ishikawa-02 (Ish-02) treated with or without TGF-β (1 ng/ml) for 4 hr. (C) Ishikawa-02 cells were first treated with TGF-β (1 ng/ml) for 10 days, and the treated cells were pooled together and named 02/D10. Q-PCR analysis (left) to measure SOX2 expression in Ishikawa-02 and 02/D10 cells. A clonogenic analysis (right) to compare the growth of Ishikawa-02 (Ish-02) to 02/D10 cells in the absence of TGF-β for 14 days. Colonies were subjected to crystal violet staining (upper) and quantified by ImageJ analysis (lower). ***P < 0.001.
