Figure 5
From: Crosstalk between SOX2 and cytokine signaling in endometrial carcinoma
SOX2 promotes migration of endometrial carcinoma cells. (A) qPCR analysis to measure SOX2 (left) and EGFR (right) expression in Ishikawa-02 (Ish-02) and Ishikawa-02-S (02-S) cells. Ishikawa-02-S is a stable clone selected from 02/D10, the pooled Ishikawa-02 cells previously treated with TGF-β (1 ng/ml) for 10 days. ***P < 0.001. (B) A clonogenic analysis to compare the growth of Ishikawa-02 (Ish-02) and Ishikawa-02-S (02-S) for 14 days. Colonies were subjected to crystal violet staining (upper) and quantified by ImageJ analysis (lower). ***P < 0.001. (C) Cell tracking analysis to monitor the trajectory (upper) and quantify migrated distance (lower) of Ishikawa-02 (Ish-02) and Ishikawa-02-S (02-S) cells under a normal serum condition. ***P < 0.001, n = 10 cells/group. (D) Cell tracking analysis to monitor the trajectory (upper) and quantify migrated distance (lower) of Ishikawa-02 cells treated with EGF (100 ng/ml) (EGF) for 24 hr under a serum starvation condition. ***P < 0.001, n = 10 cells/group.
