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Figure 2

From: Reduced Uteroplacental Perfusion Pressure (RUPP) causes altered trophoblast differentiation and pericyte reduction in the mouse placenta labyrinth

Figure 2

RUPP creates hypoxic placenta environment with altered proliferation. (A) Total Ki67 (n = 12 placentae per treatment/gestational age) and trophoblast specific Ki67 (n = 9 placentae per treatment/gestational age) and (B) pHH3+ trophoblast (k18) and pHH3+ fetal endothelial (Pecam) as measures of relative placental proliferation (n = 5 placentae per treatment/gestational age). (C) Evaluation of hypoxia (Hif-1α), TS cell populations (Eomes, Cdx2 and Tfap2c) and progenitor populations (Rhox4 and Epcam) in the E16.5 placentae by qPCR (n = 3 placentae per treatment). (D) Evaluation of the Epcam+ progenitor population by IHC (n = 3 placentae per treatment/gestational age). (E) Evaluation of hypoxia (TGF-β-3) by IHC (n ≥ 3 placentae per treatment/gestational age). Stars indicate statistical significance between the treatment groups at the identified gestational age: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

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