Figure 8

TS cells grown in hypoxic conditions see decreased GCM-1 expression as Hif-1α levels increase while the inverse occurs with Ctsq expression. (A) Gcm-1 expression peaks on Day 2 in the presence of FGF4, in both normoxic and hypoxic conditions, with expression greater in hypoxia. In the absence of FGF4, the timing of peak expression of Flt-1, Pgf and Vegfa match that of Gcm-1, with expression greater in hypoxia. (B) As Hif-1α expression increases in hypoxic conditions, Gcm-1 (Syn-T) expression decreases and Ctsq (S-TGC) increases. Ctsq expression peaks on Day 6 in the absence of FGF4, with expression highest in hypoxic conditions. Alkaline Phosphatase staining identifies S-TGC differentiation of TS cells in normoxic and hypoxic conditions in the absence of FGF4. Stars indicate statistical significance, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 (n = 3 per treatment/day). Scale bar = 100 um.