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Figure 1

From: Expression of recombinant glutamic acid decarboxylase in insect larvae and its application in an immunoassay for the diagnosis of autoimmune diabetes mellitus

Figure 1

Expression and purification of GAD65. Expression and purification in Sf9-cells (A,B), in R. nu (C,D) and in S. frugiperda (E,F). (A,C,E) SDS-PAGE (10.0% T 6.0% C, 1.5 mm, under reducing conditions, stained with Coomassie Brilliant Blue R-250); (B,D,F) WB revealed with monoclonal antibodies to either His6 or GAD65 as primary antibody. (A,C,E) Samples: 1. Total soluble fraction, 2. Unbound material, 3. Wash step, 4–7. Consecutive eluates of purified GAD65. (B,D,F) Samples: 1. Total soluble fraction, 2. Unbound material, 3. Wash step, 4–6. Consecutive eluates of purified GAD65. Arrows indicate the electrophoretic mobility of GAD65. Full-length WB are presented in Supplementary Fig. 1.

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