Figure 1

Superdex 200 gel filtration chromatograms of Trx-OsGID1/GA3 and various Trx-SLR1 mutants in isolation and together. The eluent was 20 mM sodium phosphate (pH 7.5), 150 mM NaCl, 2 mM β-ME, and 0.1 mM GA3. The protein(s) contained in the fractions indicated by the inverted open arrows and by the circled numbers were subjected to SDS-PAGE and identified by their electrophoretic migration patterns (right panel). The positions of the molecular weight standards are shown at the top of both panels.