Figure 2 | Scientific Reports

Figure 2

From: CASP4 gene silencing in epithelial cancer cells leads to impairment of cell migration, cell-matrix adhesion and tissue invasion

Figure 2

Effects of CASP4-silencing on cell-cell junctions and actin cytoskeleton polymerization. Representative confocal microscopy images of A431 cells transfected with siCTRL (A–C) or siCASP4 (D–F) stained with E-cadherin reactive antibody (red) (A,D) and phalloidin (green) (B,E) at the leading edge (a) and in the underneath confluent cell monolayer (b); merged pictures (C,F) are shown. Optical 2x zoom (A’–F’) were analyzed with the yz plane projections. Bar plots indicate the percentage of fully sealed junctions at leading edge (p = 0.0003, n = 10). E-cadherin positive junctions were analyzed in 10 confocal microscopy images recorded in two independent experiments; approximately 500 junctions were counted by using ImageJ. In panel (b) both xz and yz planes are shown. Scale bars (25 µm) are indicated. Statistical analysis was performed by Wilcoxon rank sum test for the comparison of siCASP4 with the siCTRL transfected A431 cells. Significant p-values are represented by asterisks: ***p < 0.001.

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