Figure 9

Fibronectin deposition corresponds to periostin protein peak in gingival healing in vivo. (A) Gingival tissues were dissected from the rats to assess for gene expressions of Fibronectin (Fn1). Gene expression was normalized to 18 S using the ΔΔCt method. Data represents mean fold gene expressions ± s.d. relative to control day 1 (on collagen alone) of 3 independent experiments in triplicates. Data was analyzed via one-way ANOVA (*p < 0.05; **p < 0.01). (B) Representative images of immunoreactivity for fibronectin of gingiva at 1, 3, 7, and 14 days post-wounding and unwounded gingiva (day 0). Histological sections were incubated with a primary antibody against fibronectin. The primary antibody was detected with Cy5 (red) conjugated secondary antibody. Nuclei are stained with Hoechst 33342 dye (blue). The images were taken at 10x magnification and stitched together for each tissue. Wound edge is denoted by white arrowhead. Scale bar, 50 μm.