Figure 7

Baicalein and baicalin suppress the expressions of Cox-2, iNOS, HIF-1α and VEGF. The LPS-stimulated cells were treated with LPS (1 μg/mL) for 24 hours and then challenging with various concentrations of baicalin or baicalein (3, 10, 30 nM) for another 48 hours. The protein levels of Cox-2 (~72 kDa), iNOS (~130 kDa), HIF-1α (~90 kDa) and VEGF (~27 kDa) were detected by immunoblot analysis (upper panel), and GAPDH (~38 kDa) served as an internal control. Values were shown as the percentage of LPS-induced maximum reading (lower panel), in Mean ± SEM, where n = 3. Statistically significant changes were classified as significant (*) where p < 0.05 more significant (**) where p < 0.01 and highly significant (***) where p < 0.001 as compared with control group.