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Figure 1

From: Genome-wide DNA Methylation and RNAseq Analyses Identify Aberrant Signalling Pathways in Focal Cortical Dysplasia (FCD) Type II

Figure 1

Genomic distribution of methylation changes and qPCR analysis of DNMTs in brain tissues resected from FCD type II patients. (A) Heatmap depicting Hierarchical clustering ofall samples and genomic regions according to differential methylation profiles, green methylation up (high 5mC), red methylation down (low 5mC). Clustering was done using the Hierarchical condition package in the GeneSpring GX software (version 13.0). (B) Bar chart showing genomic distribution of CpG sites with altered DNA methylation patterns in patients with FCD type II as compared to the autopsy controls. (C) mRNA levels of DNMT3α showing increased expression whereas mRNA levels of DNMT1 remain unaltered. Relative changes in gene expression were calculated using the ΔΔCq method with HPRT as a reference gene. Mean increase in transcript levels was statistically significant (*p < 0.05) with respect to the controls. Data represent mean ± SEM (n = 10), each done in triplicate.

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