Figure 2 | Scientific Reports

Figure 2

From: Genome-wide DNA Methylation and RNAseq Analyses Identify Aberrant Signalling Pathways in Focal Cortical Dysplasia (FCD) Type II

Figure 2

Integrative analysis of genes with differentially methylated promoters and differential expression and qPCR analysis of selected genes in brain tissues resected from FCD type II patients. (A,B) Venn diagram showing total number of hypermethylated/downregulated (A; left panel) and hypomethylated/upregulated genes (B; right panel) genes. A total of 104 genes were hypermethylated and downregulated in expression and 72 genes were hypomethylated and upregulated in their expression. (C) The expression level of upregulated mRNAs (RPS6KA3, PRKAA1, BRCA1, and EGFR) and downregulated mRNAs (BCL6, NTRK3, NEUROD1, and NR4A3) as identified in the RNAseq data were further validated by real-time PCR. Relative changes in gene expression were calculated using the ΔΔCq method with HPRT as a reference gene. Mean increases in transcript levels were statistically significant (*p < 0.05) with respect to control. Data represent mean ± SEM (n = 10), each done in triplicate.

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