Figure 1
From: Calponin-3 is critical for coordinated contractility of actin stress fibers
Localization of Calponin-3 in U2OS cells. (A) Endogenous Cnn3 localizes to all three stress fibers categories in U2OS cells. Examples of dorsal stress fibers are marked with red arrows, ventral stress fibers with orange arrows, and thin transverse arcs with yellow brackets. (B) Localization of endogenous Cnn3 to actin-rich (visualized by fluorescent phalloidin) stress fibers by 3D-SIM. (C) Co-localization of Cnn3-mCherry with GFP-α-actinin-1 in stress fibers as detected by 3D-SIM. The red box represents a zoomed region containing a segment of a ventral stress fiber (that is included as an inset in the top-left corner of the images). (D) The same region was analyzed with a line profile tool to illustrate the co-localization of Cnn3 to α–actinin-1 –rich regions along the stress fiber. Single slices of 3D-SIM images are presented. Scale bars, 10 µM.
