Figure 2
From: Calponin-3 is critical for coordinated contractility of actin stress fibers
Calponin-3 is a dynamic component of contractile stress fibers. (A) Cnn3 and α-actinin-1 dynamics were measured by FRAP in contractile fibers. Representative GFP-α-actinin-1 and GFP-Cnn3 expressing cells used in the experiments are shown of the left, and fluorescence recoveries of each case shown on the right. White boxes represent regions of interest (ROI). Scale bars, 10 μm. (B) Plots representing the rates of fluorescence recovery of Cnn3 and α-actinin-1 in stress fibers after photobleaching in wild-type U2OS cells. (C) The rates of α-actinin-1 fluorescence recovery in stress fibers of wild-type and Cnn3-depleted U2OS cells. n = 10 cells for all experiments. The data are presented as +/− SEM.
