Figure 3
From: Calponin-3 is critical for coordinated contractility of actin stress fibers
Calponin-3 is not critical for stress fiber assembly. (A) Actin filaments (fluorescent phalloidin) and focal adhesions (anti-vinculin) were visualized in control and Cnn3 knockout cells. The three categories of stress fibers are indicated with arrows and brackets as in Fig. 1A. Scale bars, 10 µM. (B,C) Average areas of focal adhesions (panel B) and numbers of focal adhesions per cell (panel C) were quantified from control as well as Cnn3 knockout and knockdown cells. n (cells): control (20), Cnn knockdown (19), Cnn knockout (16). Box charts have Whisker range 5–95, showing outliers. Statistical significance was assessed with Mann-Whitney test; n.s. (not-significant), p < 0.005 (*), p < 0.001 (***).
