Figure 5
From: Calponin-3 is critical for coordinated contractility of actin stress fibers
Cnn3-deficient cells exhibit increased traction forces. Composite images of live-imaging of Cnn3-knockout (A) and control (B) cells; the Tractin channel (black and white) and pillar traction forces (arrows). Green and red arrows indicate displacements above or below 30 nm, respectively. The blue X at 10 minutes indicates the time and site of stress fiber breakage in the example Cnn3-knockout cell. Scale bars, 10 μm. (C) Pillar displacements, (D) traction forces and (E) fraction of pillars displaced underneath each cell were quantified. n > 1,000 pillars per condition from 6 WT and 8 CNN3-KO cells. Bars = min – max. Student’s t-test: p < 0.05 (*); p < 0.0001 (***).
